This paper discusses about the production of biodiesel from mutton suet using Calcium Oxide mixed with Titanium Dioxide synthesized from animal (mutton) bones and Nyctanthes Arbor-Tristis leaves extract respectively, as heterogeneous catalyst. Titanium Dioxide (TiO2) was extracted by titrating Nyctanthes Arbor-Tristis leaves extract (refluxed with methanol) with titanium tetraisopropoxide whereas the Calcium Oxide (CaO) was synthesized by thermally decomposing the mutton bones. An overall conversion efficiency of 93% was recorded by following alcohol based transesterification on mutton suet. Glycerolysis reaction was carried out on suet to pretreat it at a temperature of 110oC, for 2:1 volumetric ratio of Glycerol to suet for 1 hour followed by transesterification reaction carried out at 1:3 molar ratio (suet to alcohol) for 2% catalyst concentration at 60oC .Apart from studying the reaction parameters, characteristic studies related to synthesized catalyst have been carried out.