Bovine mastitis, the most common and costly disease plaguing the dairy industry, is always a threat. It is an inflammation to the mammary gland, usually caused by a bacterial infection, generally Staphalococus aureus. Antibiotics are routinely used for the treatment of mastitis and the current antibiotic treatments are rarely effective. Farmers of the soutern region of kerala use crude extract of Marsh Barbel for the treatment of mastitis and is found to be highly effective. The present study focuss on the insilico identification and invitro analysis of  the phytochemicals which are responsible for the antimastitis activity of Marsh Barbel.

20 phytochemicals in Marsh Barbel were selected based on literature and insilico analysis was done using Schrodinger docking software with the most potent drug targets in Staphalococus aureus. From the docking results it has been observed that, out of the 20 components, Luteoline, Apigenine-7-o-glucuronide and Apigenine-7-o-glucoside were having excellent inhibitory activity towards all the five drug targets (PDB ID: 4YU3,2DGJ,5TZI,3VHR and 3VMR)  compared to the most commonly used antibiotic, Pirlimycin Hydrochloride. Invitro analysis using Staphalococus aureus strains by adopting the disc diffusion method confirmed the  antibacterial activity of all the three components.