A new HPLC process with sensitive properties was developed for synthesizing the quinolone derivative. It is accurate, precise, and robust. The solutes were estimated using a gradient C18 column of 100 mm × 4.6 mm and 2.5 μm. Quinolone derivatives were eluted with acetonitrile and water (70:30 v/v) in a 10-minute gradient trial at 1.0 ml per minute, temperature of column  25°C, and monitored at 325 nm. The synthesized combination exhibited retention times of 6.5 minutes. R2: 0.994. The Q2A and Q2B validations of the analytical technique demonstrated excellent linearity throughout concentration ranges of 10-50 μg/mL. The approach achieved remarkable accuracy and precision, both intra-day and inter-day.and outstanding robustness values. The suggested analytical technique is reliable and precise for analyzing drug combinations in bulk and tablet forms.