This research investigates the antibacterial properties of different solvent extracts derived from the freshwater algae Chara fibrosa and Cladophora crispata. The agar well diffusion technique was employed to assess their antibacterial effectiveness against three bacterial strains: E. coli, S. marcescens, and S. aureus. The zones of inhibition recorded for all crude extracts indicated a wide range of antimicrobial activity against the tested microorganisms. FTIR spectral analysis of the freshwater algal biomass confirmed the presence of organic functional groups such as C-Br, C-N, C-O, and C=C. These results highlight the potential of Chara fibrosa and Cladophora crispata biomass as a sustainable green resource. The demonstrated antibacterial activity suggests that bioactive compounds present in these freshwater algae extracts could be explored for the development of novel pharmaceutical agents with applications in both medicine and agriculture.