Numerous antimicrobial agents are available globally, yet many exhibit unexpected adverse effects on human health. This study aimed to assess the antimicrobial properties of methanolic, ethanolic, and aqueous extracts of Carica papaya seeds against human pathogens including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The seed extracts were prepared via Soxhlet extraction and their antimicrobial activity was evaluated using the agar well diffusion method, with dimethyl sulfoxide as the negative control and amoxicillin as the positive control. The results demonstrated that both methanolic and ethanolic extracts exhibited strong antimicrobial activity against all tested pathogens, whereas aqueous extracts showed comparatively lower efficacy. The highest antimicrobial activity and zone of inhibition were observed against Staphylococcus aureus. These findings suggest that Carica papaya seeds could serve as a potential source of antimicrobial agents for the treatment of bacterial infections.