The complex formed between 5-Chlorosalicylaldehyde thiosemicarbazone (CSTSC) and Pd(II) was quantitatively extracted into butyl acetate within the pH range of 3.6–4.4 after 30 seconds of equilibration. Up to a concentration of 9.0 μg mL⁻¹ Pd(II), the Pd(II)-CSTSC complex obeyed Beer’s law, with a maximum absorbance at 400 nm. Using the Job’s method of continuous variation, the stoichiometry of the Pd(II)-CSTSC complex was determined to be 1:2. The extracted complex remained stable for 96 hours. Sandell’s sensitivity was found to be 9.92 × 10⁻³ μg cm⁻², with a molar absorptivity of 1.072 × 10⁴ L mol⁻¹ cm⁻¹. The proposed method was successfully applied for analyzing synthetic mixtures, complexes, and water samples.