The objective is to evaluate the in vitro anticancer effect of epigallocatechin-3-gallate (EGCG) against the Egyptian bladder squamous cell carcinoma (SCC) cell line and elucidate its molecular mechanism.SCC cell line (SCaBER) was treated with different concentrations of EGCG at different durations (24, 48 and 72 hours). Trypan blue test was used to determine the viability of SCC cells. The expression of urothelialmarker (UPII), oncogene (EGFR), and anti-apoptotic (Surviving) were detected by real time-PCR. EGCG has shown a time and dose dependent anticancer effect on SCaBER cells.This notable anticancer effect of EGCG has been associated with down regulation of UPII and EGFR expressions as well as the decrease of the anti-apoptotic gene expression surviving. Our results have shown that EGCG exerts anti-proliferation and apoptotic induction on BC cell line in a time and dose dependent manner through the inhibition of proliferation (UPII and EGFR) and by the induction of apoptosis.